Journal:
Article Title: CD36 Regulates Oxidative Stress and Inflammation in Hypercholesterolemic CKD
doi: 10.1681/ASN.2008010009
Figure Lengend Snippet: CXCL10 levels are increased in CD36−/− mice after UUO. (Top) Representative CXCL10/IP-10 immunohistochemical photomicrographs in CD36+/+ and CD36−/− mice 7 d after UUO. (Bottom) Summary of the quantification by computer-assisted image analysis, expressed as the percentage of tubulointerstitial area staining positive for CXCL10. Data are means ± SEM. †P < 0.01, CD36+/+ versus CD36−/−. Magnification, ×400.
Article Snippet: Primary antibodies used were reactive with F4/80 (rat anti-mouse F4/80 monoclonal; AbD Serotec, Raleigh, NC), CD11b/c (rabbit anti-mouse CD11b/c; Abcam, Cambridge, MA), CXCL10 (rabbit anti-mouse CXCL10 polyclonal; PeproTech, Rocky Hill, NJ), IκB-α and phosphorylated IκB-α (rabbit anti-human IκBα polyclonal and rabbit anti-human phospho IκB-α polyclonal; Cell Signaling Technology, Danvers, MA), phosphorylated p65 (rabbit anti-mouse p65 polyclonal; Cell Signaling Technology), Smad2 (rabbit anti-human Smad2 monoclonal; Cell Signaling Technology), phosphorylated Smad2 (rabbit anti-human phospho Smad2 polyclonal; Upstate Biotechnology, Billerica, MA), and oxLDL (rabbit anti-human hypochlorous acid modified LDL polyclonal; Chemicon Int., Temecula, CA).
Techniques: Immunohistochemical staining, Staining